• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
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  • 优先权
    • 专利摘要:
    The invention relates to a surface treatment composition, wherein the composition comprises spores of at least one non-pathogenic species of the genus Bacillus and a prebiotic component. The composition can be used to inoculate the surfaces to purify the surfaces of unwanted, harmful or pathogenic microorganisms.
    公开号:BE1026988B1
    申请号:E20205048
    申请日:2020-01-23
    公开日:2020-08-24
    发明作者:Corrie Gielen；Robin Temmerman
    申请人:Chrisal；
    IPC主号:
    专利说明:

    Field of the Invention The present invention relates to compositions containing at least one prebiotic component and a probiotic component for application to surfaces. The invention also relates to a corresponding method of use thereof. Introduction It is known that in some cases traditional cleaning products and methods are not sufficient or efficient enough. For example, in hospitals, patient supplies such as furniture, floors, beds and mattresses must be cleaned regularly and thoroughly to reduce the number of pathogenic microorganisms that can affect the health of recovering patients. In addition, for obvious reasons, shared facilities, such as industrial kitchens or HVAC installations, and their components, as well as industrial facilities and components, such as filters, pumps, and ducts, also require a permanent reduction and control of such pathogenic populations.
    It is known that probiotics can be used to combat these harmful bacteria. WO 2006/125283 teaches that colonizing surfaces with non-pathogenic spore-forming bacteria via cleaning products has a beneficial effect on reducing the number of pathogenic microorganisms on such surfaces. This reduces the risk of infections and has a beneficial effect on general health.
    However, given the growing resistance of some strains of bacteria to certain cleaning products, it is important to minimize the numbers of such pathogens. There is therefore a need for products and methods that can minimize unwanted, harmful or pathogenic microorganisms on a surface.
    In addition, such compositions should be applicable to surfaces that are difficult to treat, such as surfaces that are positioned such that they are difficult to reach by conventional cleaning techniques and / or surfaces that are in ambient conditions that make them difficult to treat.
    Summary of the Invention In accordance with one aspect of the present invention, there is provided a surface treatment composition, wherein the composition comprises spores of at least one non-pathogenic species of the genus Bacillus and a prebiotic component.
    It is an advantage of the present invention that surfaces can be substantially purified from pathogenic microorganisms by inoculating the surfaces with the composition. The non-pathogenic probiotics have the effect of making nutrients on the surface less available to other microorganisms due to the mechanism of nutrient depletion. In addition, it has advantageously been found that the combination of non-pathogenic probiotics with a prebiotic component further enhances this effect. A further advantage is that both probiotic and prebiotic components have less impact on the environment compared to other compounds used in detergents, such as alcohols, etc. It is a further advantage that the composition can be used in surface purification products where the products have a shelf life of at least three years.
    In one embodiment of the composition of the present invention, the at least one non-pathogenic species of the genus Bacillus comprises at least one of Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium and Bacillus amyloliquefaciens.
    It is an advantage of this embodiment that any of the Bacillus spp. are non-pathogenic microorganisms.
    In one embodiment of the composition of the present invention, the at least one non-pathogenic species of the genus Bacillus comprises a mixture of Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium and Bacillus amyloliquefaciens, each of the four Bacillus species having a number of spores which is between 10% and 70%, with respect to the total number of spores of the four Bacillus spp.
    It is an advantage of this embodiment that the Bacillus spp. have different germination rates. As a result, Bacillus spp. have a different concentration profile over time, ensuring a constant presence of a probiotic component on the surface. In addition, this choice results in an optimized diversity of extracellular enzymes. It is a further advantage that the presence of probiotic components on the surface can be guaranteed when each of the four Bacillus species has a number of spores between 10% and 70%, with respect to the total number of spores of the four Bacillus spp. .
    In one embodiment of the composition of the present invention, the at least one non-pathogenic species of the genus Bacillus consists of a mixture of Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium and Bacillus amyloliquefaciens.
    In one embodiment of the composition of the present invention, the prebiotic component comprises at least one of a fructooligosaccharide, a galactooligosaccharide and / or inulin.
    It is an advantage of this embodiment that the prebiotic sugar further enhances the effect of the probiotic component. More specifically, it has been found that the prebiotic component accelerates the activity of the probiotic component over time and that a combination of a probiotic component and a prebiotic component according to the invention further reduces the concentration of pathogens.
    In one embodiment of the composition of the present invention, the at least one non-pathogenic species is present at a concentration of 1 x 10 ° to 1 x 1070 cfu per gram of composition.
    It is an advantage of this embodiment that the concentration range allows both surface treatment using a detergent or cleaning product comprising the composition, and surface treatment by water dosing, with products for the latter application usually having a initially higher cfu concentration per gram of composition due to the fact that these products are often diluted in use.
    In one embodiment of the composition of the present invention, the prebiotic component is present at a concentration of 100 mg to 100 g per kg of composition.
    It is an advantage of this embodiment that the enhancing effect on the probiotic component is thereby maximized.
    In one embodiment of the composition of the present invention, the spores of at least one species and the prebiotic component are at least partially microencapsulated.
    It is an advantage of this embodiment that the use of microcapsules further permits spreading germination over time, resulting in a constant presence and persistent activity of Bacillus spp. on the treated surface.
    In one embodiment of the composition of the present invention, the microcapsule comprises a friable shell and is further filled with a non-aqueous solution. In one embodiment of the composition of the present invention, the diameter of the microcapsules is 1 to 300 µm. In one embodiment of the composition of the present invention, the composition is formulated as a detergent, cleaning product, liquid, emulsion, cream, ointment, lotion, gel, oil, solution, trigger spray, aerosol spray, powder or semi-solid formulation.
    It is an advantage of this embodiment that the composition can be used as part of or formulated as the above products, thereby increasing the range of application of the composition. Advantageously, the products usually have a shelf life of at least three years.
    In one embodiment of the composition of the present invention, the surface is wood, paper or textile; the surface is an abiotic material, such as stone, metal, plastic, glass, carbon or ceramic; or is the surface a composite material.
    It is an advantage of this embodiment that the composition can be applied to a variety of surfaces, which are usually abiotic. However, with the exception of human or animal skin, this composition can also be applied to some biotic materials.
    In accordance with one aspect of the present invention, there is provided a method of treating surfaces with a surface treatment composition, the method comprising the steps of cleaning, spraying, ultrasonic nebulizing, brushing or water dosing the surfaces with the composition.
    According to one aspect of the present invention, there is provided a cleaning product comprising the surface treatment composition.
    Brief description of the figures Figure 1 shows the germination rate of probiotic and synbiotic compositions as a function of time.
    Figures 2A-2C show the effect of two cleaning products on a stone tile floor.
    Figure 3 shows the effect of cleaning products on the number of Staphylococcus aureus populations.
    Figures 4A-4D show the effect over time of ultrasonic nebulization of a synbiotic composition using probiotic counts. Detailed Description of Embodiments The invention described herein refers to a surface treatment composition, wherein the composition comprises spores of at least one species of the genus Bacillus and a prebiotic component.
    A specific use of the composition according to embodiments of the invention is a composition for the treatment of surfaces of non-living things. It is noted that the term "non-living thing" can also refer to that which used to be part of a living being, such as wood, rubber, paper and the like. However, the term "surface" here does not refer to the surface of living things, thereby excluding human skin, animal skin or fur and the like.
    More specifically, such a surface can be a surface made of or comprising wood, paper, leather, textile; the surface can be a surface made of or comprising an abiotic material, such as stone, metal (and alloys thereof), plastic and other polymeric materials, glass, ceramics, bricks and the like. Furthermore, the surface may be a composite material surface, the term "composite material" herein referring to material made of two or more constituent materials. The surface may further be a mixture of the above materials.
    In embodiments, the surface may be a surface that is exposed to air, or that is partially or completely immersed in water or another liquid.
    For the purpose of the invention, the term "surface treatment" herein refers to working on a surface for the purpose of improving or changing a condition or parameter of the surface. A specific use of the composition according to embodiments of the invention is for surface purification, which means the reduction of undesired, harmful or pathogenic surface microorganisms such as bacteria, fungi, yeasts, viruses and the like.
    For this purpose, the surface treatment may comprise the act of cleaning the surface with the composition, or by applying the composition to the surface by spraying, ultrasonic atomization, brushing or water dosing.
    For the purpose of the invention, the term "water dosing" refers herein to the purification of surfaces in installations and / or surfaces of components thereof by adding the composition to a liquid, such as a cooling liquid, a rinsing liquid or a circulating liquid, wherein the liquid is in contact with the surface to be treated. Surfaces of pools and ponds, as well as surfaces of industrial installations, such as the inner surface of pipes, pumps and filters, can be subject to biofilm growth and organic waste accumulation. The composition can thus be added to the liquid in contact with the surface.
    Where appropriate, the invention will be described with reference to such uses, without intending to limit the scope of the invention thereto. For the purpose of the present invention, the term "synbiotic" refers to a combination of a probiotic and a prebiotic. For the purpose of the present invention, the term "prebiotic" or "prebiotic component" refers to at least one of a short chain sugar (usually 3-9 monosaccharide units). In specific embodiments, the prebiotic contains one or more of the group consisting of galactooligosaccharides, fructooligosaccharides, and inulin. In preferred embodiments, the prebiotic is inulin. According to preferred embodiments of the invention, the prebiotic component has a proportion by mass that is at least 0.5 mg, preferably at least 1 mg, more preferably at least 10 mg, even more preferably at least 100 mg, and most preferably at least 10 mg. is at least 1 g, per kg of the composition. It will be understood that the prebiotic component has a proportion by mass that is at most 300 g, preferably at most 200 g, more preferably at most 100 g, and most preferably at most 80 g, per kg of the composition.
    For the purpose of the present invention, the term "probiotic" or "probiotic component" refers to spores of at least one non-pathogenic species of the genus Bacillus. In accordance with embodiments of the invention, the probiotic component comprises a mixture of spores from different non-pathogenic Bacillus species (hereinafter referred to as Bacillus spp.). In preferred embodiments of the invention, the probiotic component comprises at least one of Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium and Bacillus amyloliquefaciens. Preferably, the probiotic component comprises a mixture of spores of Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium and Bacillus amyloliquefaciens. For a mixture comprising spores of Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium and Bacillus amyloliquefaciens, each of the four Bacillus species preferably has a number of spores containing at least 10%, preferably at least 15%, and more preferably at least 20%, with respect to the total number of spores of the four Bacillus spp. in the mixture. It is further understood that each of the four Bacillus species has a spore count that is at most 70%, preferably at most 60%, more preferably at most 60%, even more preferably at most 50%, preferably at most 40% , more preferably at most 35%, and most preferably at most 30%, with respect to the total number of spores of the four Bacillus spp. in the mixture. Preferably, traces of the four species are present in substantially or exactly equal amounts in the mixture. In accordance with specific embodiments of the invention, the mixture further comprises Bacillus polymyxa.
    In accordance with specific embodiments of the invention, the blend does not include Bacillus coagulans.
    In preferred embodiments, the total amount of spores in the above mixtures ranges from 1x108 to 1x10 "° per gram of the composition. Such amount is expressed as cfu (colony forming units). It is understood that this value refers to the total number of cfu of all Bacillus spp. Together.
    In certain embodiments, the probiotic component is a mixture consisting of spores of Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium and Bacillus amyloliquefaciens.
    The transformation of spores into vegetative cells results from absorption of moisture from the surrounding surface or from the biofilm to which the preparation is applied [Knudsen et al. (2015) J. Bacteriol. 19; 198 (1): 168-77]. This moisture absorption decreases the absolute moisture content of the surface or the biofilm, thereby inhibiting the availability of moisture for other microorganisms. The vegetative Bacillus cells produce a variety of extracellular enzymes on surfaces and in biofilm that reduce the amount of organic nutrients on the surface and in biofilm [Priest (1977) Bacteriol. Rev. 41 (3): 711-53]. This reduction exerts an inhibitory effect on the availability of food to other microorganisms through nutrient depletion and the general concept of competitive exclusion [Ragione et al. (2003) Vet. Microbiol. 94 (3): 245-586].
    Treatment of a surface with a surface treatment composition containing a probiotic component can affect the concentration of unwanted and potentially harmful microorganisms.
    In a particularly interesting embodiment, such an effect can be further optimized by selecting a mixture of the above four Bacillus spp., I.e.
    Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium and Bacillus amyloliquefaciens. Because these Bacillus spp. have a different germination speed, therefore also have a different concentration distribution over time, a constant presence of the probiotic component on the surface to be treated is ensured. This choice will result in an even more pronounced fluid depletion, as well as an optimized diversity of extracellular enzymes. It has now unexpectedly been found that the addition of a prebiotic component to such a composition and its use in, for example, a cleaning product affects the concentration of pathogens on the treated surface even further. The observed effect is twofold. First, the prebiotic component accelerates the activity of the probiotic component over time. Second, the combination of a probiotic component and a prebiotic component reduces the concentration of pathogens on the treated surface even further, resulting in a lower concentration of pathogens on the treated surface compared to a comparable treatment for such a surface without the prebiotic component. The synbiotic surface treatment composition can be used to purify a surface of organic soil, which may be in the form of a biofilm matrix, as well as to reduce the number of other microorganisms such as bacteria, viruses, yeasts and fungi. The combination of prebiotics with probiotics has been found to enhance the purgative effect of the composition, with the prebiotic component appearing to support and enhance the effect of the probiotics on biofilm removal and to decrease other microorganisms.
    In accordance with embodiments of the invention, the traces of the probiotic component and the at least one prebiotic component are at least partially microencapsulated in microcapsules. For the purpose of the invention, microcapsules are particles containing a prebiotic and / or a probiotic. Such capsules are described in US 20120076864. Advantageously, the composition may be encapsulated in such microcapsules to provide the composition with a longer stability / shelf life. Furthermore, after a "free" first portion of the probiotic and prebiotic components of the composition, which portion was not microencapsulated, has already exerted the effect, the microcapsules can be delayed in releasing a second portion of the probiotic and prebiotic components. provided on the surface or in biofilm. As a result, a persistent activity of the composition up to 6 weeks can be obtained, provided that sufficient friction is present. If the preparation is thereby applied to a surface that is subject to friction (such as human or animal activity (walking, touching) or mechanical activity (interaction with machines or vehicles and the like)), the presence of the microcapsules is an established way of ensuring that the preparation performs a long-lasting action. As a result, it is advantageous to include such microcapsules in the composition for the treatment of a surface where frequent surface treatment is not possible. Typically, the microcapsules are composed of a brittle shell containing a liquid containing the spores. The shell breaks open by friction and releases the contents of the capsule. The microcapsules usually have a diameter of 1 to 300 µm.
    According to embodiments of the invention, the casing is composed of a polymer layer, such as a polymer of gelatin, polyurethane, polyolefins, polyamides, polyesters, polysaccharides, silicone resins, epoxy resins, chitosan and aminoplastic resins such as a melamine-formaldehyde resin.
    In accordance with embodiments of the invention, the probiotic and prebiotic components may be present together or separately in a microcapsule. However, microcapsules can also be provided that contain individual strains of bacteria.
    In accordance with embodiments of the invention, the liquid in the capsules is usually non-aqueous and more specifically immiscible with water (e.g., an organic oil, a silicone oil, a fluorocarbon or mixtures thereof).
    The weight ratio of the casing to the liquid contained therein is usually 1: 500 to 1: 5000. The outermost layer of this shell may contain functional reactive groups for chemical bonding.
    In accordance with embodiments of the invention, up to 10% by weight, 20% by weight, 30% by weight, 50% by weight or even 75% by weight of the prebiotic and / or probiotic component may be encapsulated in the microcapsules. Microcapsules can be dispersed in a liquid or viscous composition.
    According to embodiments of the invention, the composition is processed into cleaning products (detergents), or products for spraying, for (ultrasonic) atomization, for brushing or for water dosing. Such products are usually in liquid form.
    Furthermore, depending on the type of treatment and the type of surface in question, the composition can be used as a component in or formulated as: a detergent, a cleaning product, a liquid, an emulsion, a cream, an ointment, a lotion, a gel, an oil, a solution, a trigger spray, an aerosol spray, a powder or a semi-solid formulation. Advantageously, the composition can be incorporated into a product for atomization, preferably ultrasonic atomization, and be combined with an atomization device. An ultrasonic atomizing device herein refers to a device that uses high frequency sound vibrations to produce an extra fine water mist that is then expelled to add moisture to the chamber. Such an application has been found to permit surface treatment of surfaces that cannot be achieved with conventional cleaning techniques or that cannot be treated by means of water dosing. Surface treatment compositions for (ultrasonic) atomization treatment and methods therefor can be of particular interest for the treatment of HVAC installation surfaces, in particular the surface of the inner sides of ducts. It was found that the installation of an ultrasonic nebulizing device in the duct of an air-conditioning plant, where the device nebulized a surface treatment composition according to the invention, had a beneficial effect on the presence of harmful microorganisms. In preferred embodiments, water droplets have an average diameter ranging from 0.5 to 10 µm.
    It was found that under these conditions the composition could be maximally distributed in an available space. The invention further relates to a method of treating surfaces with a surface treatment composition as described above. Part of the composition may be microencapsulated. The method further comprises the steps of cleaning (in the sense of freeing dirt from contamination), spraying, ultrasonic atomization, brushing or water dosing of the surfaces with the composition according to methods known in the art.
    The invention further relates to a cleaning product comprising the composition as described herein. While the invention has been described above with reference to specific embodiments, this is done to illustrate and not to limit the invention, the scope of which is defined by the appended claims. Those skilled in the art will readily understand that other combinations of features than those described herein are possible without departing from the scope of the claimed invention.
    Experimental Results The invention will now be described in more detail with reference to the following examples, the purpose of which is only illustrative and is not intended to limit the scope of the invention.
    Galacto-oligosaccharides: Ergomax GOS Fructo-oligosaccharides: Supersmart Fructo Inulin: Beneo Orafti Inulin Seed rates as well as cfu count were performed using ISO 20391-1: 2018. Example 1 (E1) - Composition of a synbiotic preparation The surface treatment composition has the following components, per gram of the preparation: - 2x 10 ° spores of each of the following species: Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium and Bacillus amyloliquefaciens; - 50 mg inulin; 25 mg galactooligosaccharides; - 15 mg of fructooligosaccharides; - 5 mg microcapsules.
    Comparative Example 2 (CE2) - Composition of a probiotic preparation
    The surface treatment composition has the following components, per gram of the preparation: 2x 10 ° spores of each of the following species: Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium and Bacillus amyloliquefaciens; - 5mg microcapsules.
    Example 3 (E3) - Effect of a prebiotic component A sample from Example 1 (E1) and a sample from Comparative Example 2 (CE2) were each diluted in water at a dilution ratio of 1: 100 and then allowed to germinate.
    Figure 1 shows the germination rate of both samples.
    From this figure it can be concluded that germination of the probiotic component takes place faster in a synbiotic formulation.
    In other words, the inclusion of a prebiotic component in a product containing a probiotic component results in an increased presence of the probiotic component in the product when evaluated in shorter time periods. Example 4 (E4) - Comparative Examples 5-6 (CE5-CE6) - Cleaning product A universal cleaning product was defined, having one of the following compositions, as summarized in Table 1: Table 1 - Cleaning product compositions Sodium Laureth Sulfate 55 (55 55 | Galacto-oligosaccharide 025 0 (9 | where the components of the cleaning product are expressed by weight and where the Probiotic Mixture is an aqueous mixture of Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium and Bacillus amyloliquefaciens. Each component of the probiotic mixture is present in a concentration of 2 x 10 ° cfu per gram of the total composition, which means that each component of the mixture has a number of spores that is 25% with respect to the total number of spores Example 5 (E7) - Purification of a surface Cleaning products according to composition E4 and CE5 were used to make stone tile floors of toilets of a private experimental room under controlled conditions. The stone tile floors suffered from severe organic contamination and biofilm, especially in the concrete joints between the tiles.
    An image of a stone tile floor before treatment is shown in Figure 2A.
    A first of such floors was cleaned with a universal cleaning product with a composition according to CE5. A second of the stone tile floors was cleaned with a universal cleaning product with a composition according to E4, which is a synbiotic composition.
    The first and second floors were then cleaned daily for a period of three weeks, with the cleaning product based on CE5 and E4, respectively.
    FIG. 2B shows the result after three weeks of daily cleaning with the (probiotic) cleaning product with a composition according to CE5. The probiotic cleaning product was able to remove superficial biofilm on the tile surface and partially in the joints. FIG. 2C shows the result after three weeks of daily cleaning with the (synbiotic) E4 cleaning product. The synbiotic cleaning product was able to remove much more organic dirt and biofilm deep in the joints between the tiles, making them clear in most places, indicating that the addition of a prebiotic component to a probiotic cleaning product had an unexpected stimulating effect. has. Example 6 (E8) - Purification of a surface Universal cleaning products according to composition E4 (synbiotic composition), CE5 (probiotic composition) and CE6 (which forms a dilute detergent) were applied daily on a floor in a confined experimental room under controlled conditions for a total duration of 10 days.
    Microbiological samples were taken from the surface of the floor to determine the presence of the hospital bacterium Staphylococcus aureus. Such samples were taken at 1, 6, 24, 48, 72, 120 and 240 hours. From 24 hours onwards, samples were taken just prior to product application to measure the effect of the previous 24 hours of application.
    The results in Figure 3 show the following: Application of the diluted detergent ("control" cleaning product based on composition CE6) does not appear to affect the level of S. aureus CFUs. Application of the probiotic cleaning product ("probiotic" - composition CES cleaning product) results in a drop in S. aureus CFUs from 24 hours to reach a minimum level (i.e. 430 cfu / m2) after 240 hours.
    Application of the synbiotic cleaning product ("synbiotic" - based on composition E4) leads to a more rapid decline of S. aureus CFUs resulting in a lower level (i.e. 220 cfu / m2) after 240 hours.
    Example 7 (E9) - Ultrasonic Nebulization of Synbiotic Preparation The synbiotic preparation as described in E1 was diluted ten-fold in water containing 0.1% by weight of Sodium Laureth Sulfate. The dilution was then introduced into an ultrasonic nebuliser. A Bionaire® BUL9500B-U Warm and Cool Mist Ultrasonic Humidifier device, a commercially available humidifier, was used for this. A fog was generated with particles having a diameter predominantly between 0.8 µm and µm. The facility was located in one corner of a room of 120 m placed and sprayed for 20 seconds every 15 minutes, at an output of 1 ml of synbiotic solution per minute. Agar nutrient plates were placed on a table at the opposite end of the chamber, with lids open. Every 15 minutes, after each nebulization, one agar nutrient plate was closed and incubated. A probiotic count was determined as such at the start point (Fig. 4A) and after 15 (Fig. 4B), 30 (Fig. 4C) and 60 (Fig. 4D) minutes.
    As illustrated by Fig. 4A-4D, a high probiotic count was measured as early as 30 minutes, indicating that ultrasonic nebulization provides a very effective distribution of probiotics through the air.
    权利要求:
    Claims (14)
    [1]
    A surface treatment composition, wherein the composition comprises spores of at least one non-pathogenic species of the genus Bacillus and a prebiotic component.
    [2]
    The surface treatment composition according to claim 1, wherein the at least one non-pathogenic species of the genus Bacillus comprises at least one of Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium and Bacillus amyloliquefaciens.
    [3]
    A surface treatment composition according to claim 1 or claim 2, wherein the at least one non-pathogenic species of the genus Bacillus comprises a mixture of Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium and Bacillus amyloliquefaciens, wherein each of the four Bacillus species has a spore count between 10% and 70%, with respect to the total spore count of the four Bacillus spp.
    [4]
    A surface treatment composition according to any preceding claim, wherein the at least one non-pathogenic species of the genus Bacillus is a mixture of Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium and Bacillus amyloliquefaciens.
    [5]
    A surface treatment composition according to any of the preceding claims, wherein the prebiotic component comprises at least one of a fructooligosaccharide, a galactooligosaccharide and / or inulin.
    [6]
    A surface treatment composition according to any preceding claim, wherein the at least one non-pathogenic species is present at a concentration of 1 x 10 ° to 1 x 1019 cfu per gram of composition.
    [7]
    A surface treatment composition according to any one of the preceding claims, wherein the prebiotic component is present at a concentration of 100 mg to 100 g per kg of composition.
    [8]
    Surface treatment composition according to any one of the preceding claims, wherein the spores of at least one species and the prebiotic component are at least partially microencapsulated.
    [9]
    The surface treatment composition of claim 8, wherein the microcapsule comprises a friable shell and is additionally filled with a non-aqueous solution.
    [10]
    The surface treatment composition of claim 8 or 9, wherein the diameter of the microcapsules is 1 to 300 µm.
    [11]
    A surface treatment composition according to any preceding claim, wherein the composition is formulated as a detergent, cleaning product, liquid, emulsion, cream, ointment, lotion, gel, oil, solution, trigger spray, aerosol spray, powder, or semi-solid formulation. .
    [12]
    Surface treatment composition according to any of the preceding claims, wherein the surface is wood, paper or textile; wherein the surface is an abiotic material, such as stone, metal, plastic, glass, carbon or ceramic; or wherein the surface is a - composite material.
    [13]
    A method of treating surfaces with a surface treatment composition according to any one of claims 1 to 12, the method comprising the steps of cleaning, spraying, ultrasonic nebulizing, brushing or water dosing the surfaces with the composition.
    [14]
    A cleaning product comprising the surface treatment composition of any of claims 1 to 13.
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    同族专利:
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    法律状态:
    2020-10-12| FG| Patent granted|Effective date: 20200824 |
    优先权:
    申请号 | 申请日 | 专利标题
    EP19153404.9A|EP3686266A1|2019-01-23|2019-01-23|Synbiotic surface treatment composition|
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